Cultures
We are using novel optical imaging techniques to study dynamics of networks of dissociated rat cortical neurons. We use voltage sensitive dyes to record rapid changes in cell membrane potential corresponding to the generation of an action potential. We are currently investigating neural network dynamics of spontaneous electrical activity from these cultures and am using our measure to analyze the temporal behavior of the networks as the network matures over time. Future work will involve the introduction of pharmacological agents to modulate changes in network communication.
Featured Results
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Network of cultured cortical neurons: Optical imaging of the activity of interconnected cells. Application of voltage sensitive dyes and a fast CCD camera allow us to monitor activity of every neuron in the field of view of the microscope.
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A multi-electrode array (MEA). This measurement probe allows us to simultaneously record activity in 64 locations in a culture with high time resolution. It also enables electrical stimulation (Picture from MultiChannel Systems). |
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